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Further study will help us to understand the function of ApNAC1 in andrographolide biosynthesis. ApNAC1 was heterologously expressed in Escherichia coli and recombinant protein was purified by Ni-NTA agarose.
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Based on its expression pattern, ApNAC1 gene might involve in andrographolide biosynthesis. Compared with control sample, its expression abundance sharply increased with methyl jasmonate treatment. The analysis of real-time quantitative PCR revealed that ApNAC1 gene predominantly expresses in leaves. The results indicated that ApNAC1 is a nucleus-localized protein. To confirm the subcellular localization, ApNAC1-GFP was transiently expressed in A.
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The analysis of bioinformatics showed that the gene encodes a peptide of 323 amino acids, with a predicted relative molecular weight of 35.9 kDa and isoelectric point of 6.14. The coding sequence of ApNAC1 was cloned by RT-PCR, and GenBank accession number was KY196416. paniculata transcriptomic data, the expression pattern of one member of NAC transcription factor family ( ApNAC1) matched with andrographolide accumulation. paniculata leaves with MeJA treatment was performed. To investigate the underlying andrographolide biosynthesis mechanisms, RNA-seq for A. Wang, Jian Qi, Meng-Die Guo, Juan Shen, Ye Lin, Hui-Xin Huang, Lu-QiĪndrographis paniculata is widely used as medicinal herb in China for a long time and andrographolide is its main medicinal constituent.